JARID1B Antibody 3273S from Cell Signaling Technology

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Cancer Science
April 01 2019

Fig 1: miR‐381‐3p and miR‐486‐5p overexpression impairs JARID1B mRNA translation without affecting JARID1B mRNA levels and upregulates the JARID1B target gene BRCA1. A, Representative western blotting image of JARID1B protein and H3K4me3 levels in cell lysate from MCF7 cells 24 h after transfection with control pSP65/U1, pSP65/U1‐miR‐381‐3p or pSP65/U1‐miR‐486‐5p. B and C, Relative quantification of JARID1B protein (B) and H3K4me3 (C) levels in western blotting analysis, n = 7. Data are represented as the mean ± SD of JARID1B or H3K4me3 levels relative to actin. Statistical significance was assessed according to 2‐tailed paired Student's t test, **P < .01. D and E, Quantitative RT‐PCR analysis of JARID1B (D) and BRCA1 (E) mRNA levels from MCF7 cells 48 and 72 hours after transfection with control pSP65/U1, pSP65/U1‐miR‐381‐3p or pSP65/U1‐miR‐486‐5p, n = 4. JARID1B and BRCA1 raw data (Ct) were normalized on GAPDH expression levels (ΔCt) as endogenous control and ΔΔCt was calculated with respect to control transfection (pSP65/U1). Data are represented as the mean ± SD. Statistical significance was assessed according to 2‐tailed Student's t test, **P < .01. F, ChIP assay (see Materials and Methods) to test JARID1B association and H3K4 trimethylation level at the BRCA1 promoter. The location of the primers used for the PCR amplification of the BRCA1 proximal promoter segment is reported in the map at the top. The ACTB exon V region is used as a negative control for JARID1B binding and H3K4 tri‐methylation
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Oncology Letters
May 02 2018

Fig 2: JARID1B activates PTEN transcription by decreasing H3K4me3. (A) Western blot analysis of the proteins, H3K4me3, H3K9me3 and H3K27me3, in UACC-462 cells with silencing of JARID1B. (B) Western blot analysis of the proteins, H3K4me3, H3K9me3 and H3K27me3, in AsPC-1 cells with overexpression of JARID1B. (C) The predicted binding region of H3K4me3 to the PTEN promoter. (D) ChIP-qPCR was performed to assess H3K4me3 occupancy in UACC-462 cells with silent expression of JARID1B. (E) qChIP was performed in order to assess H3K4me3 occupancy in AsPC-1 cells overexpressing JARID1B. **P<0.01. Error bars represent the standard deviation. JARID1B, Jumonji AT-rich interactive domain 1B; PTEN, phosphatase and tensin homolog; qChIP, quantitative chromatin immunoprecipitation.
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PloS one
January 01 2011

Fig 3: Jarid1b associates with the promoters of E2f-target genes during senescence.(A) Analysis of cycling, passage 5 (P5) and senescent, passage 8 (P8), primary MEFs for hallmarks of senescence. Shown are a proliferation curve of MEFs according to the 3T4 protocol (left panel, results shown as means ± SD), β–galactosidase staining of P5 and P8 MEFs (center panel) and quantification of β–galactosidase positive cells (right panel). (B) Analysis of mRNA expression of the indicated genes from P5 and P8 MEFs. Values are normalized to P5 and shown as means ± SD. (C) Jarid1b ChIP in P5 MEFs and P8 MEFs. The degree of enrichment at indicated promoters of E2f-target genes and control genes was measured by qPCR, non-specific binding of rabbit IgG controls was subtracted and results are presented as percentage of bound/input normalized to P5 samples for each gene. (D) H3K4me3 ChIP in P5 MEFs and P8 MEFs, performed as in (C). Non-specific binding of rabbit IgG controls was subtracted and quantification of H3K4me3 samples was normalized to H3-immunoprecipitations in the same experiment. PD: passage doubling, P: passage.
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Cancer Science
April 01 2019

Fig 4: JARID1B/KDM5B, miR‐381‐3p and miR‐486‐5p expression level in breast cancer patients and association of their expression level with patients’ survival. A, Box plot showing miR‐381‐3p, miR‐486‐5p and JARID1B/KDM5B expression in normal (blue) and cancer (yellow) subsets. Statistical significance was assessed according to 2‐tailed paired Student's t test, ****P < .0001. B, Scatter plot of miR‐381‐3p vs JARID1B/KDM5B expression in both normal (blue) and cancer (yellow) samples. Pearson coefficients are calculated for total (ρ = −.40), normal‐only (ρN = .40) and cancer‐only (ρC = .02) datasets and respective correlation lines (black, blue, yellow) are reported in the plot. C, Scatter plot for miR‐486‐5p vs JARID1B/KDM5B expression in both normal (blue) and cancer (yellow) samples. Pearson coefficients are calculated for total (ρ = −.72), normal‐only (ρN = −.65) and cancer‐only (ρC = −.14) datasets and respective correlation lines (black, blue and yellow) are reported in the plot. D, Kaplan‐Meier diagram showing survival for patients with higher (>50th percentile, cyan) or lower (<50th percentile, magenta) expression of miR‐381. E, Kaplan‐Meier diagram showing survival for patients with higher (>50th percentile, cyan) or lower (<50th percentile, magenta) expression of miR‐486. F, Kaplan‐Meier diagram showing survival for patients with higher (>50th percentile, cyan) or lower (<50th percentile, magenta) expression of JARID1B
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Cancer Science
April 01 2019

Fig 5: Expression of JARID1B protein and mRNA and of miR‐486‐5p in different human breast cancer cell lines. A, Representative western blotting image of JARID1B protein levels in the indicated breast cancer cell lines. B, Real time RT‐PCR quantitation of miR‐486‐5p in the indicated breast cancer cell lines. The histogram reports the amounts relative to the U6 endogenous calibrator. Data are the average of 3 experiments; SD is indicated. C, Real‐time RT‐PCR quantitation of JARID1B mRNA in the indicated breast cancer cell lines. The histogram reports the amounts relative to the GADPH endogenous calibrator. Data are the average of 3 experiments; SD is indicated

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Cell Signaling Technology for
JARID1B Antibody

Cell Signaling Technology's JARID1B Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Precipitation, Immunoprecipitation, and Western Blot. The JARID1B Antibody was generated using KDM5B as the antigen and it reacts with Homo Sapiens, Human, and Non-Human Primate.

Description

JARID1B Antibody detects endogenous levels of JARID1B protein. The antibody does not cross-react with other JARID proteins, including JARID1A, JARID1C and JARID1D

Product Specs
ItemJARID1B Antibody
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number3273S
Quantity100 µl
ApplicationsW, IP
ReactivityH, Mk
TargetJARID1B
HostRabbit
NCBI Gene AliasesCT31, JARID1B, MRT65, PLU-1, PLU1, PPP1R98, PUT1, RBBP2H1A, RBP2-H1
NCBI Full Gene Namelysine demethylase 5B
ClonalityPolyclonal
Research AreaChromatin Regulation / Nuclear Function
Molecular Weight180

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(1) (R)-2-Hydroxyglutarate Inhibits KDM5 Histone Lysine Demethylases to Drive Transformation in IDH-Mutant CancersCancer DiscoveryJune 2, 2023Kathryn Gunn, Matti Myllykoski, John Z. Cao, Manna Ahmed, Bofu Huang, Betty Rouaisnel, Bill H. Diplas, Michael M. Levitt, Ryan Looper, John G. Doench, et al.Cat# 3876, RRID:AB_2129055), rabbit polyclonal anti-KDM5B antibody (Cell Signaling Technology Cat# 3273, RRID:AB_1264191), rabbit polyclonal anti-KDM5C antibodies (Abcam Cat# ab34718, RRID:AB_881090 and Abcam

(2) A druggable copper-signalling pathway that drives inflammationNatureMay 1, 2023Solier S, Müller S, Cañeque T, Versini A, Mansart A, Sindikubwabo F, Baron L, Emam L, Gestraud P, Pantoș GD, et al.(Cell Signaling, 3876S, clone D28B10, lot 5, WB 1:1,000, Hu, Ms), KDM5B (also known as Jarid1b) (Cell Signaling, 3273T, lot 3, WB 1:1,000, Hu), KDM5B (Abcam, ab181089, clone EPR12794, WB 1:1,000, Ms, KO validated by manufacturer),

(3) Inhibition of KDM5A attenuates cisplatin-induced hearing loss via regulation of the MAPK/AKT pathwayCell Mol Life SciNovember 17, 2022Chang Liu, Zhiwei Zheng, Wen Li, Dongmei Tang, Liping Zhao, Yingzi He, Huawei LiAbcam), KDM5A rabbit monoclonal antibody (1:500, 3876; CST), KDM5B rabbit polyclonal antibody (1:500, 3273; CST), KDM5C rabbit monoclonal antibody (1:500, 5361; CST), H3K4me3 rabbit monoclonal antibody (1:500,

(4) Human CPTP promotes growth and metastasis via sphingolipid metabolite ceramide and PI4KA/AKT signaling in pancreatic cancer cellsInternational journal of biological sciencesJanuary 1, 2022Zhang Y, Ji S, Zhang X, Lu M, Hu Y, Han Y, Shui G, Lam SM, Zou X.ProteinTech Group (Wuhan, China), anti-p-AKT Thr308 (cat. No. 9275) and anti- KDM5B (JARID1B; cat. No. 3273) from Cell Signaling Technology, anti-PI4KA (cat. No. ab111565; Abcam), anti-SH3BP1 (cat. No. TA811580S)

(5) Dietary glutamine supplementation suppresses epigenetically-activated oncogenic pathways to inhibit melanoma tumour growthNature CommunicationsDecember 1, 2020Mari B. Ishak Gabra et al.1:2000, JARID1A (Cell Signaling Tech 3876 monoclonal, clone# D28B10) 1:1000, JARID1B (Cell Signaling Tech 3273) 1:1000, JARID1C (Cell Signaling Tech 5361 monoclonal, clone# D29B9) 1:1000, and β-actin (Sigma A1978

(6) Prospective study of midline abdominal incisional hernia repair by component separation technique augmented with prosthetic meshInternational Surgery JournalSeptember 23, 2020Ravi Saroha, Shivani B. Paruthy, Sunil SinghJournal | October 2020 | Vol 7 | Issue 10 Page 3274 Saroha R et al. Int Surg J. 2020 Oct;7(10):3273-3279 RESULTS wound discharge, skin sutures were partly removed and a dressing was applied. From October

(7) Molecular mechanisms of Guadecitabine induced FGFR4 down regulation in alveolar rhabdomyosarcomasNeoplasiaJuly 1, 2020Emad Darvishi, Katherine Slemmons, Zesheng Wan, Sheetal Mitra, Xiaogang Hou, Jean Hugues Parmentier, Yong-Hwee Eddie Loh, Lee J. HelmanBSA in TBST). After 1 hour, cells were labeled with anti-KDM5B (1:100 dilution; Cell signaling, cat. #3273) primary antibody overnight followed by Cy3-conjugated goat anti-rabbit (111-165-045; Jackson ImmunoResearch,

(8) Modifications of H3K4 methylation levels are associated with DNA hypermethylation in acute myeloid leukemiaThe FEBS JournalMarch 1, 2020Stefania Scalea, Carmen Maresca, Caterina Catalanotto, Rachele Marino, Carlo Cogoni, Anna Reale, Michele Zampieri, Giuseppe Zardo(Cell Signaling Technology #2148; Danvers, MA, USA), polyclonal rabbit anti-human JARID1B (Cell Signaling Technology #3273; Danvers, MA, USA), polyclonal rabbit anti-human EZH2 (Abcam #ab186006; Cambridge, MA, USA) polyclonal

(9) Small Molecule Inhibitors of KDM5 Histone Demethylases Increase the Radiosensitivity of Breast Cancer Cells Overexpressing JARID1BMoleculesMay 4, 2019Simone Pippa et al.Cell Signaling Technology, RRID:AB_2118009), rabbit anti-JARID1B (1:50, Cell Signaling Technology, Cat#3273 RRID: AB_1264191). Goat-anti-Rabbit Horseradish Peroxidase conjugated (1:10,000, #31460, Thermo Fisher Scientific

(10) JARID 1B expression and its function in DNA damage repair are tightly regulated by mi RNA s in breast cancerCancer ScienceApril 1, 2019Ivano Mocavini, Simone Pippa, Valerio Licursi, Paola Paci, Daniela Trisciuoglio, Cecilia Mannironi, Carlo Presutti, Rodolfo NegriDiagnostics). Filters were hybridized with rabbit anti‐JARID1B (1:200, Cell Signaling Technology, Cat#3273 RRID: AB_1264191), rabbit anti‐actin (1:1000, Sigma‐Aldrich, Cat#A2066 RRID: AB_476693), rabbit anti‐H3K4me3

(11) Multiplicity among solar-type starsAstronomy & AstrophysicsNovember 1, 2018J.-L. Halbwachs, M. Mayor, S. Udry3497 164922 165341 166620 0.80 1.26 0.97 0.84 0.79 1.10 1.04 1.30 1.36 1.06 0.78 0.77 1.13 1.30 1.00 0.81 0.90 0.95 0.92 0.97 0.96 0.87 0.77 0.80 0.95 1.22 0.86 0.81 1.20 1.17 0.77 1.37 0.82 1.02 0.99 1.00 0.63 1.16 0.87 1.16 1.26 0.86 0.86 1.16 1.36 0.76 0.96 1.10 0.77 0.95 1.18 0.80 0.86 0.87 −77.89 42.47 −32.32 −31.42 −14.59 26.73 −1.73 −84.79 −69.43 −53.64 −61.49 −69.54 −112.42 −19.10 −26.65 7.47 7.56 −67.15 −65.10 36.76 23.43 −13.88 18.37 18.50 7.08 −36.54 −31.13 −12.95 −11.16 −9.37 9.57 −32.07 2.33 −5.61 −6.65 −7.15 83.21 34.02 −59.79 3.15 2.23 0.44 0.10 −0.09 −23.89 −38.56 21.68 −24.41 23.88 −13.14 −29.78 20.14 −9.73 −19.47 0.12 0.07 0.11 0.04 0.09 0.05 0.08 0.12 0.09 0.32 0.14 0.06 0.13 0.15 0.07 0.20 0.07 0.09 1.00 0.09 0.11 0.09 0.11 0.19 0.10 0.13 0.10 0.09 0.13 0.12 0.11 0.09 0.12 0.14 0.09 0.09 0.09 0.08 0.12 0.13 0.15 0.10 0.13 0.08 0.07 0.08 0.05 0.12 21.35 0.17 0.17 0.07 0.52 0.07 0.35 0.35 0.25 11.19 0.28 0.29 0.36 0.43 0.52 1.33 0.45 0.23 0.22 0.32 0.28 21.20 0.31 0.13 5.37 19.12 0.35 0.26 0.37 0.63 0.32 0.13 0.28 0.18 0.27 0.33 0.20 0.26 0.29 0.31 0.33 0.27 4.10 0.30 0.11 0.21 0.32 0.25 0.40 0.28 0.26 0.28 4.27 0.36 6.23 0.47 0.48 0.22 2.16 0.22 0.88 0.93 0.68 38.02 0.87 1.00 0.97 0.80 1.17 3.63 1.10 0.74 0.50 0.77 0.85 66.80 0.98 0.47 11.08 32.80 1.00 0.90 1.01 1.81 0.87 0.34 0.85 0.62 0.72 0.82 0.61 0.79 0.86 0.87 1.02 0.83 11.36 0.86 0.34 0.55 0.77 0.81 1.27 0.93 0.74 0.87 14.16 1.02 18.63 1.29 1.24 0.75 7.03 0.74 0.676 0.720 0.916 0.000 0.719 0.465 0.563 0.806 0.100 0.000 0.290 0.962 0.992 0.783 0.816 0.000 0.507 0.992 0.000 0.000 0.452 0.614 0.424 0.000 0.699 0.998 0.704 0.952 0.824 0.759 0.934 0.855 0.639 0.620 0.404 0.790 0.000 0.779 0.997 0.952 0.768 0.751 0.113 0.620 0.958 0.727 0.000 0.410 0.000 0.114 0.152 0.911 0.000 0.911 11 34 11 82 14 41 21 12 32 17 10 23 11 8 22 110 18 10 29 25 11 10 11 11 13 8 11 11 8 11 9 15 8 7 15 15 38 18 8 8 8 10 10 15 22 16 38 9 105 8 8 17 17 16 11 34 11 82 14 41 21 12 32 17 10 23 11 8 22 120 18 10 58 47 11 10 11 11 13 8 11 11 8 11 9 15 8 7 15 15 38 18 8 8 8 10 10 15 22 16 38 9 105 8 8 17 17 16 2904 5721 3291 6669 4025 7364 7272 5092 5500 5161 3689 7337 4091 2676 4892 6013 6014 5830 6651 2973 2991 6291 5812 5812 5225 2994 4849 3803 2963 4546 3339 3619 2674 3033 4847 4847 5246 6307 2655 2643 2643 3273 3273 3694 4477 4821 4436 2561 4369 2618 2226 3717 5911 4092 CST CST CST SB1O CST CST CST CST CST SB1 CST CST CST CST CST SB2O CST CST SB2 SB2O CST CST CST SB14 CST CST CST CST CST CST CST CST CST CST CST CST SB1O CST CST CST CST CST CST CST CST CST SB1O CST SB1O5 CST CST CST SB16 CST + + * + + A81,

(12) Jumonji Inhibitors Overcome Radioresistance in Cancer through Changes in H3K4 Methylation at Double-Strand BreaksCell reportsOctober 23, 2018Bayo J, Tran TA, Wang L, Peña-Llopis S, Das AK, Martinez ED.AB_1977252 Anti-53BP1,Cell Signaling Technology,Cat#4937; RRID: AB_106954558 Anti-KDM5B,Cell Signaling Technology,Cat#3273; RRID: AB_1264191 Anti-KDM4B,Cell Signaling Technology,Cat#D7E6; RRID: AB_11140642 Anti-mouse IgG-HRP linked

(13) Jumonji AT‑rich interactive domain 1B promotes the growth of pancreatic tumors via the phosphatase and tensin homolog/protein kinase B signaling pathwayOncology LettersMay 2, 2018Xudong Shen, Guilian Cheng, Liming Xu, Wei Wu, Zhengrong Chen, Peng DuDanvers, MA, USA) against Actin (1:2,000; cat. no. CST 3700S), anti-JARID1B (1:1,000; cat. no. CST 3273T), anti-PTEN (1:1,000; cat. no. CST 9188T), anti-protein kinase B (Akt) (1:1,000; cat. no. CST 2920S),

(14) Cross-talk between human airway epithelial cells and 3T3-J2 feeder cells involves partial activation of human MET by murine HGFPloS oneJanuary 1, 2018Hynds RE, Gowers KHC, Nigro E, Butler CR, Bonfanti P, Giangreco A, Prêle CM, Janes SM.

(15) The HIF/PHF8/AR axis promotes prostate cancer progressionOncogenesisDecember 19, 2016Tong D, Liu Q, Liu G, Yuan W, Wang L, Guo Y, Lan W, Zhang D, Dong S, Wang Y, et al.

(16) Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cellsOncotargetFebruary 2, 2016Xu YM, Wu DD, Zheng W, Yu FY, Yang F, Yao Y, Zhou Y, Ching YP, Lau AT.

(17) MiR137is an androgen regulated repressor of an extended network of transcriptional coregulatorsOncotargetNovember 3, 2015Emeli M. Nilsson, Kristian B. Laursen, Jonathan Whitchurch, Andrew McWilliam, Niels Ødum, Jenny L. Persson, David M. Heery, Lorraine J. Gudas, Nigel P. MonganKDM4A/ 35720 Oncotarget JMJD2A (Cell Signaling Technology, Danvers, MA; #3393; 1:500), KDM5B/JARID1B (Cell Signaling; #3273; 1:1000), KDM7A/JHDM1D (Sigma-Aldrich, Gillingham, UK; #SAB2101190; 1 µg/ml), MED1/TRAP220 (R&D Systems,

(18) Histone demethylase KDM5B collaborates with TFAP2C and Myc to repress the cell cycle inhibitor p21(cip) (CDKN1A)Molecular and cellular biologyMay 1, 2012Wong PP, Miranda F, Chan KV, Berlato C, Hurst HC, Scibetta AG.The following antibodies for Western blotting (Santa Cruz unless stated otherwise) were used: KDM5B (3273; Cell Signaling), Myc tag (2276; Cell Signaling), p53 (p53-DO-1, sc-126), TFAP2C (6E4, sc53162), p21cip

(19) The histone demethylase Jarid1b (Kdm5b) is a novel component of the Rb pathway and associates with E2f-target genes in MEFs during senescencePloS oneJanuary 1, 2011Nijwening JH, Geutjes EJ, Bernards R, Beijersbergen RL.recovered from immune complexes on protein A-agarose beads with the following antibodies: Jarid1b (#3273, Cell Signaling Technology), H3K4me3 (ab1012, Abcam), H3 (#4620, Cell Signaling Technology) and normal rabbit IgG

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Cancer Science

Oncology Letters

PloS one

Cancer Science

Cancer Science

JARID1B Antibody from Cell Signaling Technology

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Cell Signaling Technology

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JARID1B Antibody from Cell Signaling Technology

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